使用方法: 1. Thaw the Reagent. It should take approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C, to completely thaw the 20ml size. 2. Pipet 20μl of Reagent into each well of the 96-well assay plate containing the samples in 100μl of culture medium. Note: We recommend repeating pipettes, digital pipettes or multichannel pipettes for convenient delivery of uniform volumes of Reagent to the 96-well plate. 3. Incubate the plate at 37°C for 1–4 hours in a humidifi ed, 5% CO2 atmosphere. Note: To measure the amount of soluble formazan produced by cellular reduction of MTS, proceed immediately to Step 4. Alternatively, to measure the absorbance later, add 25μl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidifi ed chamber at room temperature for up to 18 hours. Proceed to Step 4. 4. Record the absorbance at 490nm using a 96-well plate reader.