参考文献: 1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42. 3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74. 4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92.
Gene IDs: 20307
纯度: > 97 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of rMuMCP-2/CCL8 as determined by LAL method.
活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.