参考文献: 1. Conlon MA, Tomas FM, Owens PC, et al. 1995. J Endocrinol, 146: 247-53. 2. Dunaiski V, Dunshea FR, Walton PE, et al. 1997. J Endocrinol, 155: 559-65. 3. Garnaut SM, Howarth GS, Read LC. 2002. Growth Factors, 20: 17-25. 4. Xi G, Kamanga-Sollo E, Pampusch MS, et al. 2004. J Cell Physiol, 200: 387-94. 5. Hadsell DL, Parlow AF, Torres D, et al. 2008. J Endocrinol, 198: 61-70.
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 50 EU/mg of Recombinant HumanLR3 IGF-1 as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.