参考文献: 1. Matsuo H, Kangawa K, Minamino N. 1988. Tanpakushitsu Kakusan Koso, 33: 2438-50. 2. Uehara Y, Shimizu H, Shimomura Y, et al. 1990. Neuropeptides, 17: 107-10. 3. Hunt PJ, Espiner EA, Nicholls MG, et al. 1997. Peptides, 18: 1475-81. 4. Richards AM, Lainchbury JG, Nicholls MG, et al. 2002. Trends Endocrinol Metab, 13: 151-5. 5. Trojnarska O, Gwizdala A, Katarzynski S, et al. 2010. Int J Cardiol, 139: 241-7.
Gene IDs: 4879
纯度: > 97 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant HumanBNP as determined by LAL method.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.