参考文献: 1. Shamir A, Kwon OB, Karavanova I, et al. 2012. J Neurosci, 32: 2988-97. 2. Malek RL, Toman RE, Edsall LC, et al. 2001. J Biol Chem, 276: 5692-9. 3. Zhang Z, Prentiss L, Heitzman D, et al. 2006. J Neurosci Res, 84: 1-12. 4. Law AJ, Shannon Weickert C, Hyde TM, et al. 2004. Neuroscience, 127: 125-36.
Gene IDs: 3084
纯度: > 96 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant HumanNRG1-β2 as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.