参考文献: 1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8. 2. Belas RandFlaherty D. 1994. Gene, 148: 33-41. 3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53. 4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94. 5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.
Gene IDs: 1253480
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of rFlagellin, His as determined by LAL method.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.