参考文献: 1. Prendergast FG, Mann KG. 1978. Biochemistry. 17:3448-53. 2. Shaner NC, Steinbach PA, Tsien RY. 2005. Nat Methods. 2:905-9. 3. Phillips GJ. 2001. FEMS Microbiol Lett. 204:9-18. 4. Wong FH, Banks DS, Abu-Arish A, et al. 2007. J Am Chem Soc. 129:10302-3.
Gene IDs: 7011691
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of reGFP as determined by LAL method.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.