参考文献: 1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9. 2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31. 3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
Gene IDs: 116637
来源: Escherichia coli.
纯度: > 95 % by SDS-PAGE and HPLC analyses.
内毒素水平: Less than 1 EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.
活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.