参考文献: 1. Banwell ME, Tolley NS, Williams TJ, et al. 2002. Cytokine, 17: 317-23. 2. Kagami S, Saeki H, Komine M, et al. 2005. Clin Exp Immunol, 141: 459-66. 3. Stubbs VE, Power C, Patel KD. 2010. Immunology, 130: 74-82. 4. El-Shazly AE, Doloriert HC, Bisig B, et al. 2013. Clin Exp Allergy, 43: 322-31.
氨基酸序列: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L
Gene IDs: 10344
纯度: > 97 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant HumanEotaxin-3/CCL26 as determined by LAL method.
活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR3 transfected HEK293 cells is in a concentration range of 0.5-2.0 μg/ml.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.