参考文献: 1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52. 2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43. 3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53. 4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.
Gene IDs: 5008
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant HumanOSM as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.