参考文献: 1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6. 2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2. 3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601. 4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80. 5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904. 6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.
Gene IDs: 25647
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of rRtIL-7 as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.