参考文献: 1. Schmutz J, Martin J, Terry A, et al. 2004. Nature, 431: 268-74. 2. Wynn TA. 2003. Annu Rev Immunol, 21: 425-56. 3. Heinzmann A, Mao XQ, Akaiwa M, et al. 2000. Hum Mol Genet, 9: 549-59. 4. Wang M, Xing ZM, Lu C, et al. 2003. Hum Genet, 113: 387-90.
内毒素水平: Less than 1 EU/μg of Recombinant HumanIL-13, Variant as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with 5 % trehalose.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.