参考文献: 1. Yoon C, Johnston SC, Tang J, et al. 2000. EMBO J. 19:3530-41. 2. Oppmann B, Lesley R, Blom B, et al. 2000. Immunity. 13:715-25.
Gene IDs: 3592
纯度: > 95 % by SDS-PAGE and HPLC analyses.
内毒素水平: Less than 1 EU/μg of Recombinant HumanIL-12 as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.