参考文献: 1. Walter M, Nyman D, Krajnc V, et al. 1977. Thromb Haemost, 38: 438-46. 2. Aronson DL. 1976. Thromb Haemost, 36: 9-13. 3. Sugai K, Imamura Y, Uechi S, et al. 1986. Yakugaku Zasshi, 106: 335-42. 4. Hu X, Sun Y, Chen Z. 2000. Zhonghua Shao Shang Za Zhi, 16: 241-3.
纯度: > 97 % by SDS-PAGE and HPLC analyses.
来源: Pichia pastoris
内毒素水平: Less than 0.5 EU/mg of rBX as determined by LAL method.
活性: Fully biologically active when compared to standard. The biologically activity of rRpBX is no less than 500 KU/mg (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37 oC in vitro within 60 ± 20 seconds.
形态: Lyophilized from a 0.2 μm filtered concentrated sodium acetate buffer solution.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.