参考文献: 1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65. 2. Barao I. 2012. Front Immunol, 3: 402. 3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27. 4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97.
Gene IDs: 3604
纯度: > 97 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant Human4-1BB Receptor as determined by LAL method.
活性: Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 μg for both 4-1BB Ligand and 4-1BB Receptor.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
形态: Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.