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Gene IDs: 650
纯度: > 95 % by SDS-PAGE and HPLC analyses.
来源: Escherichia coli.
内毒素水平: Less than 1 EU/μg of Recombinant HumanBMP-2 as determined by LAL method.
活性: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 103 IU/mg.
形态: Lyophilized from a 0.2 μm filtered concentrated solution containing 10 mM sodium citrate pH 3.5.
溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.
储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.