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文献标题：Polypeptide sono-adjuvant for ultrasound-activatable regulation of innate immunity and cancer vaccination therapy

发表期刊：Nature Communications (IF=15.7)

DOI：https://doi.org/10.1038/s41467-025-66976-2

使用 Absin 产品：Mouse IL-6 ELISA Kit（货号：abs520004）

Fig. 1: US-triggered mechanical and oxidative force activated STING, TLR9 and NLRP3 pathways in APCs in vitro.
a Schematic illustration showing US-triggered mechanical force stimulated innate immune activation in DCs. b The intracellular Ca2+ concentration of DC2.4 cells upon US stimulation (n?=?3 independent experiments). c Representative CLSM images to show the intracellular Ca2+ concentration of DC2.4 cells after indicated power density of US stimulation (n?=?3 independent experiments). Scale bar?=?10?μm. d The intracellular Ca2+ concentration of DC2.4 cells at indicated time points after US stimulation (n?=?3 independent experiments). e The intracellular Ca2+ concentration of DC2.4 cells after US stimulation with or without the addition of Dooku-1 (n??=?3 independent experiments). f Western blot assay of US-triggered NLRP3 activation in DC2.4 cells or RAW264.7 cells (n?=?3 independent experiments). g Heat-map of US-triggered IL-1β secretion in BMDCs (n?=?3 independent experiments). h Heat-map of US-triggered active caspase-1 release in BMDCs (n?=?3 independent experiments). i US-triggered active caspase-1 release in BMDCs with or without the addition of Dooku-1 (n?=?3 independent experiments). j Schematic illustration for the preparation of SONP and mechanism of US-triggered oxidative force to stimulate innate immune activation. k Representative CLSM images of intracellular ROS generation in DC2.4 cells after SONP?+?US treatment (n?=?3 independent experiments). Scale bar?=?10?μm. l Western blot assay of endoplasmic reticulum stress induced by SONP?+?US in DC2.4 cells. I: PBS, II: SONP, III: SONP?+?US (n?=?3 independent experiments). m Representative CLSM images of mitochondrial DNA release in DC2.4 cells after SONP?+?US treatment (n?=?3 independent experiments). Scale bar?=?10?μm. n Western blot assay of US-triggered STING and TLR9 activation in DC2.4 cells (n?=?3 independent experiments). o IL-6 and TNF-α secretion by BMDCs after SONP?+?US treatment (n?=?3 independent experiments). p The ISG response produced by RAW264.7-ISG-Luc or THP-1-ISG-Blue cells after SONP?+?US treatment (n?=?3 independent experiments). Unless specified otherwise, the data are presented as mean?±?SD. Statistical significance was calculated via one-way ANOVA with Tukey’s test (b, e, i, o) and P values were indicated. Source data are provided as a Source Data file. The elements in this figure (a, j) were created by Adobe Illustrator.