产地 | 上海 |
品牌 | 爱必信(absin) |
货号 | abs923 |
保存条件 | 25°C可稳定保存2周; 4°C稳定保存3个月; 如长期贮存,需置于-20°C保存,有效期18个月,避免反复冻融 |
用途 | Western blot |
包装规格 | 500ul,5*500ul |
保质期 | 25°C可稳定保存2周; 4°C稳定保存3个月; 如长期贮存,需置于-20°C保存,有效期18个月,避免反复冻融 |
是否进口 | 否 |
产品描述 |
|
描述 |
本产品是一种三色预染蛋白标准品,含12种高度纯化预染的蛋白质,涵盖10至245kD的各种分子量。以Tris-glycine buffer体系为例,其中25kD条带为绿色条带,75kD条带为红色条带,其余条带为蓝色。用于Western过程中显示转膜(PVDF、 NC或者nylon膜都适用)是否成功以及显示蛋白的分子量大小。本产品已经配制在上样缓冲液中,可直接使用。每种蛋白的浓度约为0.1~0.4 mg/ml,贮存缓冲液为:20 mM Tris-phosphate, pH 7.5 at 25°C),2 % SDS, 0.2 mM Dithiothre itol, 3.6 M Urea, and 15 % (v/v) Glycerol。
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形式 |
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
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操作步骤 |
1.使用前请室温静置融化;
2.温和充分混匀,取本产品5μl与实验样品同时进行SDS-PAGE电泳。 3.未使用的彩虹预染marker按照储存条件要求保存。 |
操作说明 |
1.本产品可直接使用,不需加热。
2.及时更换电泳缓冲液并使用新配制的凝胶,以免影响电泳结果。 3.本产品4°C可稳定保存3个月,如需保存更长时间,请-20°C保存。 4.为避免污染,开封后,可根据需要分装成小管,建议每管分装10μl,-20℃贮存,每次取一管使用,避免反复冻融。 |
质量控制 |
Under suggested conditions, Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.
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基本信息 |
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别名 |
Protein Marker;蛋白marker,预染蛋白marker ,彩虹蛋白marker ,
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储存/保存方法 |
25°C可稳定保存2周; 4°C稳定保存3个月; 如长期贮存,需置于-20°C保存,有效期18个月,避免反复冻融。
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实验步骤 | 推荐指数 | 货号 | 产品名称 | 规格 |
样本制备 |
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abs9228 | RIPA (中) | 100ml |
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abs9229 | RIPA (强) | 100ml | |
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abs9230 | RIPA (弱) | 100ml | |
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abs9231 | RIPA (强,无抑制剂) | 100ml | |
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abs9160 | NP-40 | 100ml | |
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abs9195 | 1M Tris-HCl,pH6.8 | 1L | |
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abs9201 | 1M Tris-HCl,pH8.8 | 1L | |
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abs9117 | SDS | 100ml | |
★★ | abs9116 | WB/IP 细胞裂解液 | 100ml | |
★★ | abs9239 | WB/IP 细胞裂解液(无抑制剂) | 100ml | |
★★★★ | abs9101 | 红细胞裂解液 | 100ml,250ml,500ml | |
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abs812852 | PMSF | 50mg,100mg,200mg | |
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abs816442 | Aprotinin 抑肽酶 | 10mg,25mg,50mg,100mg | |
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abs817902 | Peptatin 胃蛋白酶抑制剂A | 10mg,25mg,50mg,100mg | |
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abs9133 | EDTA | 100ml | |
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abs42017684 | EGTA | 1g,10g | |
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abs817431 | 原钒酸盐 | 100mg,500mg | |
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abs47014865 | β-甘油磷酸盐 | 10g,25g | |
★★ | abs9161 | 广谱型蛋白酶抑制剂混合物 | 1ml | |
★★ | abs9162 | 广谱型磷酸酶抑制剂混合物 | 1ml | |
蛋白定量 | ★★ | abs9232 | BCA蛋白定量试剂盒 | 500次,2500次 |
★★ | abs9233 | 增强型BCA 蛋白定量试剂盒 | 500次 | |
上样与电泳 |
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abs9226 | DTT溶液(1mol/L) | 5ml,10ml |
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abs9237 | 2X Laemmli样品缓冲液 | 5ml*2 | |
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abs9238 | 10%SDS十二烷基硫酸钠溶液 | 500ml | |
★★ | abs953 | 2*上样缓冲液 (适用于SDS-PAGE) | 10ml | |
★★ | abs9301 | 预制胶 10%,10 wells | 5片/盒 | |
★★ | abs9308 | 预制胶 10%,15 wells | 5片/盒 | |
★★ | abs9302 | 预制胶 12%,10 wells | 5片/盒 | |
★★ | abs9309 | 预制胶 12%,15 wells | 5片/盒 | |
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abs9303 | 预制胶 15% ,10 wells | 5片/盒 | |
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abs9310 | 预制胶 15% ,15 wells | 5片/盒 | |
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abs9304 | 预制胶 4-15%, 10 wells | 5片/盒 | |
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abs9311 | 预制胶 4-15%, 15 wells | 5片/盒 | |
★★ | abs9305 | 预制胶 4-20%, 10 wells | 5片/盒 | |
★★ | abs9312 | 预制胶 4-20%, 15 wells | 5片/盒 | |
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abs9307 | 预制胶 8%, 15 wells | 5片/盒 | |
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abs9306 | 预制胶 8-20%, 10 wells | 5片/盒 | |
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abs9313 | 预制胶 8-20%, 15 wells | 5片/盒 | |
NEW | abs9328 | 3-5kd小分子量预制胶,16.5%,10wells | 10片/盒 | |
NEW | abs9329 | 3-5kd小分子量预制胶,16.5%,15wells | 10片/盒 | |
★★★ | abs925 | 蛋白电泳套装 | 盒 | |
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abs951 | running buffer | 100ml,1L | |
★★★★★ | abs923 | 预染蛋白marker, 10-245 KDa | 500ul,5*500ul | |
★★★★★ | abs924 | 预染蛋白marker, 10-180 kDa | 250ul,2*250ul | |
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abs966 | Genshare考马斯亮蓝蛋白胶快速染色液 | 500ml | |
★★★ | abs964 | 考马斯亮蓝染色试剂盒(常规型)R250 | 1kit | |
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abs965 | 考马斯亮蓝染色试剂盒(增强型)G250 | 1kit | |
转膜 |
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abs931 | PVDF膜0.2 μm | 20张 |
★★ | abs932 | PVDF膜0.45 μm | 20张 | |
★★ | abs959 | NC膜0.2 μm | 20张 | |
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abs960 | NC膜0.45 μm | 20张 | |
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abs950 | 10*电转液 | 100ml,1L | |
封闭 |
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abs9174 | 脱脂奶粉 | 100g,500g |
★★★★★ | abs9155 | BSA | 50g,100g | |
一抗孵育 | ★★ | abs830031 | Beta actin antibody | 50ul,100ul |
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abs132001 | Beta actin antibody | 50ug,100ug | |
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abs119600 | Beta Actin Polyclonal Antibody | 50ul,100ul | |
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abs132184 | Beta-Actin antibody | 50ug,100ug | |
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abs137975 | Beta-Actin Mouse Monoclonal Antibody | 50ug,100ug | |
★★ | abs830030 | GAPDH antibody | 50ul,100ul | |
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abs132004 | GAPDH antibody | 50ug,100ug | |
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abs137959 | GAPDH Mouse Monoclonal Antibody | 50ug,100ug | |
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abs118936 | GAPDH Polyclonal Antibody | 50ul,100ul | |
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abs131993 | Tubulin alpha Antibody | 50ug,100ug | |
★★ | abs830032 | Tubulin Beta antibody | 50ul,100ul | |
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abs131994 | Tubulin beta Antibody | 50ug,100ug | |
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abs125209 | Tubulin Beta Polyclonal Antibody | 50ul,100ul | |
二抗孵育 | ★★ | abs20001 | Goat anti-mouse IgG-HRP | 100ul,500ul,1ml |
★★ | abs20002 | Goat anti-rabbit IgG-HRP | 100ul,500ul,1ml | |
★★ | abs20003 | Goat anti-Mouse IgG-FITC | 1ml,500ul,100ul | |
★★ | abs20004 | Goat anti-Rabbit IgG-FITC | 100ul,500ul,1ml | |
★★ | abs20005 | Rabbit anti-Goat IgG-HRP | 100ul,500ul,1ml | |
一抗孵育 |
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abs830001 | Anti- GST | 50ul,100ul |
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abs830002 | Anti- His Tag抗体 | 50ul,100ul | |
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abs830003 | Anti- His(C-terminal Specific) 抗体 | 50ul,100ul | |
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abs830004 | Anti- Myc 抗体 | 50ul,100ul | |
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abs830005 | Anti- DYKDDDDK | 50ul,100ul | |
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abs830006 | Anti- HA 抗体 | 50ul,100ul | |
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abs830008 | Anti-V5,mAb | 50ul,100ul | |
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abs830010 | Anti- GST,HRP | 50ul | |
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abs830011 | Anti- His Tag (HRP) 抗体 | 50ul | |
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abs830012 | Anti- His(C-terminal Specific) (HRP) 抗体 | 50ul | |
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abs830013 | Anti- Myc (HRP) 抗体 | 50ul | |
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abs830014 | Anti- DYKDDDDK,HRP | 50ul | |
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abs830015 | Anti- HA (HRP) 抗体 | 50ul | |
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abs830016 | Anti- V5 (HRP) 抗体 | 50ul | |
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abs137956 | C-Myc-Tag Mouse Monoclonal Antibody | 50ug,100ug | |
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abs137958 | Flag-Tag Mouse Monoclonal Antibody | 50ug,100ug | |
显色 | ★★★★★ | abs920 | ECL化学发光检测试剂盒 | 2*25ml,2*50ml,2*250ml |
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abs9212 | DAB 显色试剂盒(棕黄色,WB) | 1kit | |
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abs9213 | DAB 显色试剂盒(紫蓝色,WB) | 1kit | |
其他辅助试剂 |
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abs952 | 10*TBST | 100ml,1L |
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abs9152 | Tween-20 | 500ml | |
★★ | abs954 | WB专用一抗二抗稀释液 | 100ml | |
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abs969 | Western一抗二抗去除液(中性) | 250ml | |
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abs968 | Western一抗二抗去除液(弱碱性) | 250ml | |
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abs967 | Western一抗二抗去除液(强碱性) | 250ml | |
新手* | ★★★★★ | abs926 | WB solution base kit(rabbit) | 1kit |
★★★★★ | abs927 | WB solution base kit(mouse) | 1kit | |
★★★★★ | abs928 | WB solution base kit(goat) | 1kit |
产品更多信息请进入爱必信网站咨询 |