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产品详请
| 产地 | 上海 |
| 品牌 | 爱必信(absin) |
| 货号 | abs9232 |
| 用途 | Western blot |
| 包装规格 | 500次 |
| 纯度 | % |
BCA法是理想的蛋白质定量方法，在碱性环境下蛋白质分子中肽键结构与Cu2+络合并将Cu2+还原成Cu1+。BCA特异地与Cu1+结合形成稳定的紫蓝色复合物，在562nm处有*的光吸收值并与蛋白质浓度成正比，颜色的深浅与蛋白质的含量成正比，可以根据吸收值测定蛋白质浓度。该测定方法灵敏度高，操作简单，试剂及其形成的颜色复合物稳定性俱佳，并且受干扰物质去垢剂等影响小。
产品特点：
1、准确灵敏，线性范围广，BCA试剂的蛋白质测定范围是20~2000ug/mL；
2、快速：45min内完成测定，比经典的Lowry法快4倍而且更加方便；
3、不受样品中离子型和非离子型去污剂影响；
4、检测不同蛋白质分子的变异系数远小于考马氏亮蓝,可以酶标板法或分光光度计法测定蛋白质浓度。
组分：
| 品名 | 500次 | 2500次 |
| BCA蛋白定量A液 | 100mL | 125mL×4 |
| BCA蛋白定量B液 | 4mL | 10mL×2 |
| 5mg/mL BSA | 1mL | 1mL×5 |
WB更多配套试剂推荐：
| 实验步骤 | 推荐指数 | 货号 | 产品名称 | 规格 |
| 样本制备 | abs9228 | RIPA (中) | 100ml | |
| abs9229 | RIPA (强) | 100ml | ||
| abs9230 | RIPA (弱) | 100ml | ||
| abs9231 | RIPA (强，无抑制剂) | 100ml | ||
| abs9160 | NP-40 | 100ml | ||
| abs9195 | 1M Tris-HCl，pH6.8 | 1L | ||
| abs9201 | 1M Tris-HCl，pH8.8（更多pH请看官网） | 1L | ||
| abs9117 | SDS | 100ml | ||
| ★★ | abs9116 | WB/IP 细胞裂解液 | 100ml | |
| ★★ | abs9239 | WB/IP 细胞裂解液(无抑制剂) | 100ml | |
| ★★★★ | abs9101 | 红细胞裂解液 | 100ml,250ml,500ml | |
| abs812852 | PMSF | 50mg,100mg,200mg | ||
| abs816442 | Aprotinin 抑肽酶 | 10mg,25mg,50mg,100mg | ||
| abs817902 | Peptatin 胃蛋白酶抑制剂A | 10mg,25mg,50mg,100mg | ||
| abs9133 | EDTA | 100ml | ||
| abs42017684 | EGTA | 1g,10g | ||
| abs817431 | 原钒酸盐 | 100mg,500mg | ||
| abs47014865 | β-甘油磷酸盐 | 10g,25g | ||
| ★★ | abs9161 | 广谱型蛋白酶抑制剂混合物 | 1ml | |
| ★★ | abs9162 | 广谱型磷酸酶抑制剂混合物 | 1ml | |
| 蛋白定量 | ★★ | abs9232 | BCA蛋白定量试剂盒 | 500次,2500次 |
| ★★ | abs9233 | 增强型BCA 蛋白定量试剂盒 | 500次 | |
| 上样与电泳 | abs9226 | DTT溶液(1mol/L) | 5ml,10ml | |
| abs9237 | 2X Laemmli样品缓冲液 | 5ml*2 | ||
| abs9238 | 10%SDS十二烷基硫酸钠溶液 | 500ml | ||
| ★★ | abs953 | 2*上样缓冲液 (适用于SDS-PAGE) | 10ml | |
| ★★ | abs9301 | 预制胶 10%,10 wells | 5片/盒 | |
| ★★ | abs9308 | 预制胶 10%,15 wells | 5片/盒 | |
| ★★ | abs9302 | 预制胶 12%,10 wells | 5片/盒 | |
| ★★ | abs9309 | 预制胶 12%,15 wells | 5片/盒 | |
| abs9303 | 预制胶 15% ,10 wells | 5片/盒 | ||
| abs9310 | 预制胶 15% ,15 wells | 5片/盒 | ||
| abs9304 | 预制胶 4-15%, 10 wells | 5片/盒 | ||
| abs9311 | 预制胶 4-15%, 15 wells | 5片/盒 | ||
| ★★ | abs9305 | 预制胶 4-20%, 10 wells | 5片/盒 | |
| ★★ | abs9312 | 预制胶 4-20%, 15 wells | 5片/盒 | |
| abs9307 | 预制胶 8%, 15 wells | 5片/盒 | ||
| abs9306 | 预制胶 8-20%, 10 wells | 5片/盒 | ||
| abs9313 | 预制胶 8-20%, 15 wells | 5片/盒 | ||
| NEW | abs9328 | 3-5kd小分子量预制胶，16.5%，10wells | 10片/盒 | |
| NEW | abs9329 | 3-5kd小分子量预制胶，16.5%，15wells | 10片/盒 | |
| ★★★ | abs925 | 蛋白电泳套装 | 盒 | |
| abs951 | running buffer | 100ml,1L | ||
| ★★★★★ | abs923 | 预染蛋白marker, 10-245 KDa | 500ul,5*500ul | |
| ★★★★★ | abs924 | 预染蛋白marker, 10-180 kDa | 250ul,2*250ul | |
| abs966 | Genshare考马斯亮蓝蛋白胶快速染色液 | 500ml | ||
| ★★★ | abs964 | 考马斯亮蓝染色试剂盒(常规型)R250 | 1kit | |
| abs965 | 考马斯亮蓝染色试剂盒(增强型)G250 | 1kit | ||
| 转膜 | abs931 | PVDF膜0.2 μm | 20张 | |
| ★★ | abs932 | PVDF膜0.45 μm | 20张 | |
| ★★ | abs959 | NC膜0.2 μm | 20张 | |
| abs960 | NC膜0.45 μm | 20张 | ||
| abs950 | 10*电转液 | 100ml,1L | ||
| 封闭 | abs9174 | 脱脂奶粉 | 100g,500g | |
| ★★★★★ | abs9155 | BSA | 50g,100g | |
| 一抗孵育 | ★★ | abs830031 | Beta actin antibody | 50ul,100ul |
| abs132001 | Beta actin antibody | 50ug,100ug | ||
| abs119600 | Beta Actin Polyclonal Antibody | 50ul,100ul | ||
| abs132184 | Beta-Actin antibody | 50ug,100ug | ||
| abs137975 | Beta-Actin Mouse Monoclonal Antibody | 50ug,100ug | ||
| ★★ | abs830030 | GAPDH antibody | 50ul,100ul | |
| abs132004 | GAPDH antibody | 50ug,100ug | ||
| abs137959 | GAPDH Mouse Monoclonal Antibody | 50ug,100ug | ||
| abs118936 | GAPDH Polyclonal Antibody | 50ul,100ul | ||
| abs131993 | Tubulin alpha Antibody | 50ug,100ug | ||
| ★★ | abs830032 | Tubulin Beta antibody | 50ul,100ul | |
| abs131994 | Tubulin beta Antibody | 50ug,100ug | ||
| abs125209 | Tubulin Beta Polyclonal Antibody | 50ul,100ul | ||
| 二抗孵育 | ★★ | abs20001 | Goat anti-mouse IgG-HRP | 100ul,500ul,1ml |
| ★★ | abs20002 | Goat anti-rabbit IgG-HRP | 100ul,500ul,1ml | |
| ★★ | abs20003 | Goat anti-Mouse IgG-FITC | 1ml,500ul,100ul | |
| ★★ | abs20004 | Goat anti-Rabbit IgG-FITC | 100ul,500ul,1ml | |
| ★★ | abs20005 | Rabbit anti-Goat IgG-HRP | 100ul,500ul,1ml | |
| 一抗孵育 | abs830001 | Anti- GST | 50ul,100ul | |
| abs830002 | Anti- His Tag抗体 | 50ul,100ul | ||
| abs830003 | Anti- His(C-terminal Specific) 抗体 | 50ul,100ul | ||
| abs830004 | Anti- Myc 抗体 | 50ul,100ul | ||
| abs830005 | Anti- DYKDDDDK | 50ul,100ul | ||
| abs830006 | Anti- HA 抗体 | 50ul,100ul | ||
| abs830008 | Anti-V5,mAb | 50ul,100ul | ||
| abs830010 | Anti- GST,HRP | 50ul | ||
| abs830011 | Anti- His Tag (HRP) 抗体 | 50ul | ||
| abs830012 | Anti- His(C-terminal Specific) (HRP) 抗体 | 50ul | ||
| abs830013 | Anti- Myc (HRP) 抗体 | 50ul | ||
| abs830014 | Anti- DYKDDDDK,HRP | 50ul | ||
| abs830015 | Anti- HA (HRP) 抗体 | 50ul | ||
| abs830016 | Anti- V5 (HRP) 抗体 | 50ul | ||
| abs137956 | C-Myc-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
| abs137958 | Flag-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
| 显色 | ★★★★★ | abs920 | ECL化学发光检测试剂盒 | 2*25ml,2*50ml,2*250ml |
| abs9212 | DAB 显色试剂盒（棕黄色，WB） | 1kit | ||
| abs9213 | DAB 显色试剂盒（紫蓝色，WB） | 1kit | ||
| 其他辅助试剂 | abs952 | 10*TBST | 100ml,1L | |
| abs9152 | Tween-20 | 500ml | ||
| ★★ | abs954 | WB专用一抗二抗稀释液 | 100ml | |
| abs969 | Western一抗二抗去除液（中性） | 250ml | ||
| abs968 | Western一抗二抗去除液（弱碱性） | 250ml | ||
| abs967 | Western一抗二抗去除液（强碱性） | 250ml | ||
| 新手* | ★★★★★ | abs926 | WB solution base kit（rabbit） | 1kit |
| ★★★★★ | abs927 | WB solution base kit（mouse） | 1kit | |
| ★★★★★ | abs928 | WB solution base kit（goat） | 1kit |
爱必信特色产品线(全部现货)： 二抗、标签抗体、对照抗体；WB相关：ECL发光液、预染marker、预制胶；IHC相关：二抗试剂盒、组化笔；IP/CoIP试剂盒；细胞因子、信号通路调节剂；血清、BSA、蛋白酶K、CTB、TTX、CEE；凋亡试剂盒、呼吸爆发试剂盒；动植物提取物，定*务(抗体/多肽/蛋白)... 产品更多信息请进入爱必信网站咨询 |
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