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产地 | 上海 |
品牌 | 爱必信(absin) |
货号 | abs42017010 |
用途 | A fluorescent probe commonly used for determination of peroxynitrite & other ROSs |
包装规格 | 5mg |
纯度 | % |
CAS编号 | 109244-58-8 |
是否进口 | 否 |
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荧光染色试剂描述: 中文名称:DHR 123; 2-(3,6-Diamino-9H-xanthene-9-yl)-benzoic acid methyl ester 英文名称:Dihydrorhodamine 123 CAS:109244-58-8 纯度: 外观: 分子量:346.38 分子式:无 C21H18N2O3 光谱特性: 产品描述: Dihydrorhodamine 123 (DHR 123) is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. It is not fluorescent until oxidized by ROS to the highly fluorescent product Rhodamine 123 . Neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DHR, rather, these ROS are thought to combine with other cellular components such as cytochrome c oxidase or Fe2+ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123. Formation of Rhodamine 123 can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1). 应用:A fluorescent probe commonly used for determination of peroxynitrite & other ROSs 保存方法:Store at -20° C 溶解性:Soluble in ethanol (~10 mg/ml), DMSO (~10 mg/ml), DMF (~10 mg/ml), PBS(pH7.2) (<20 µg/ml), chloroform, and methanol. Insoluble in water. mdl号:MFCD00285712 产品信息订购:
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