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产品描述:

产品名称:Recombinant Human IL-2

氨基酸序列: APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

参考文献: 1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.<br/>2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.<br/>3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.<br/>4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.<br/>5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.

Accession: <a href="http://www.uniprot.org/uniprot/P60568" target="_blank">P60568</a>

中文别名: IL-2蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/3558" target="_blank">3558</a>

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1.0 EU/μg of Recombinant HumanIL-2 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 3.5.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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