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产品描述:

产品名称:Recombinant Human bFGF

氨基酸序列: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

参考文献: 1.Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2.Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3.Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4.Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5.Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6.Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7.Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

Accession: <a href="http://www.uniprot.org/uniprot/P09038" target="_blank">P09038</a>

中文别名: bFGF蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/2247" target="_blank">2247</a>

来源: Escherichia coli.

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanbFGF as determined by LAL method.

活性: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is ≤10 ng/ml.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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