公告提醒:爱必信所有产品和服务仅用于科学研究,不用于临床应用及其他用途提供产品和服务(也不为任何个人提供产品和服务)!

产品描述:

产品名称:Recombinant Mouse 5'-Nucleotidase/NT5E/CD73 (C-6His)

氨基酸序列: WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTI WFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISG LFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHS GFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKY LGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQ TCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLP FGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRV PIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRI KFHHHHHH

背景说明: Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity.CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

英文别名: 5'-nucleotidase,Ecto-5'-nucleotidase,CD73,5'-NT

描述: Recombinant Mouse 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus.

Accession: Q61503

中文别名: 5'-Nucleotidase蛋白; 5'-NT蛋白; NT5E蛋白; CD73蛋白

来源: Human Cells

纯度: Greater than 95% as determined by reducing SDS-PAGE.

内毒素水平: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

活性: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is 20000-80000 pmol/min/μg.

形态: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.

储存/保存方法: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

产品信息订购:

产品更多信息请进入爱必信网站咨询