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产品描述:

产品名称:Recombinant Enhanced Green Fluorecence Protein

氨基酸序列: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

参考文献: 1.Prendergast FG, Mann KG. 1978. Biochemistry. 17:3448-53.<br/>2.Shaner NC, Steinbach PA, Tsien RY. 2005. Nat Methods. 2:905-9.<br/>3.Phillips GJ. 2001. FEMS Microbiol Lett. 204:9-18.<br/>4.Wong FH, Banks DS, Abu-Arish A, et al. 2007. J Am Chem Soc. 129:10302-3.

Accession: <a href="http://www.uniprot.org/uniprot/P42212" target="_blank">P42212</a>

中文别名: Green Fluorecence蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/7011691" target="_blank">7011691</a>

种属: Invertebrate

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of reGFP as determined by LAL method.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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