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产品描述:

产品名称:Recombinant Human Nectin-4/PVRL4 (C-6His)

氨基酸序列: GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEG RVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEE GQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVS HPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPS GVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVVVDHHH HHH

背景说明: Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

英文别名: PVRL4/Nectin-4/Ig superfamily receptor LNIR/Poliovirus receptor-related protein 4/PRR4/LNIR

描述: Recombinant Human Nectin-4 is produced by our Mammalian expression system and the target gene encoding Gly31-Val351 is expressed with a 6His tag at the C-terminus.

Accession: Q96NY8

中文别名: Nectin-4蛋白; PVRL4蛋白

来源: Human Cells

纯度: Greater than 95% as determined by reducing SDS-PAGE.

内毒素水平: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

活性: Measured by the ability of the immobilized protein to support the adhesion of NIH?3T3 mouse embryonic fibroblast cells.When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour incubation at 37 °C.Elisa:000000Immobilized Anti-Human Nectin-4 mAb at 2μg/ml (100 μl/well) can bind Human Nectin-4-His .The ED50 of Human Nectin-4-His is 42.5 ng/ml.Immobilized Human Nectin-4-His at 5μg/ml (100 μl/well) can bind Human Nectin-1-His .The ED50 of Human Nectin-1-His is 1.33 ug/ml.

形态: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

溶解方法: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

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