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产品描述:

产品名称:Recombinant Mouse LIGHT/TNFSF14

氨基酸序列: DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV

中文别名: LIGHT蛋白; HVEM-L蛋白; TNFSF14蛋白; CD258蛋白

来源: Escherichia coli.

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rMuLIGHT/TNFSF14 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using human HT-29 cells is less than 2 μg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and Recombinant HumanIFN-γ.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAc to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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