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产品描述:

产品名称:Recombinant Human Endostatin

氨基酸序列: NYEKPALHLA ALNMPFSGDI RADFQCFKQA RAAGLLSTYR AFLSSHLQDL STIVRKAERY SLPIVNLKGQ VLFNNWDSIF SGHGGQFNMH IPIYSFDGRD IMTDPSWPQK VIWHGSSPHG VRLVDNYCEA WRTADTAVTG LASPLSTGKI LDQKAYSCAN RLIVLCIENS FMTDARK

参考文献: 1.HT Ren, H Hu, Y Li, et al. 2013. J Zhejiang Univ Sci B, 14: 224-30.<br/>2.W Zhuo, Y Chen, X Song, et al. 2011. Front Med, 5: 336-40.<br/>3.G HuangandL Chen. 2009. Cancer Biother Radiopharm, 24: 589-96.<br/>4.DM Wei, Y Gao, XR Cao, et al. 2006. Biochem Biophys Res Commun, 345: 1398-404.<br/>5.B Brankin, M Campbell, P Canning, et al. 2005. Exp Eye Res, 81: 22-31.

Accession: <a href="http://www.uniprot.org/uniprot/P39059" target="_blank">P39059</a>

中文别名: Endostatin蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/1306" target="_blank">1306</a>

种属: Human

来源: Escherichia coli.

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanEndostatin as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 µg/mL in the presence of Anti-Human Endostatin Polyclonal Antibody.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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