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产品描述:

产品名称:Recombinant Rat IL-1RA

氨基酸序列: HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ

参考文献: 1.Dinarello CA. 1994. FASEB J. 8:1314-25.<br/>2.Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6.<br/>3.Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7.<br/>4.Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94.<br/>5.Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.

Accession: <a href="http://www.uniprot.org/uniprot/P25086" target="_blank">P25086</a>

中文别名: IL-1RA蛋白; IL-1F3蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/60582" target="_blank">60582</a>

种属: Rat

来源: Escherichia coli.

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 10³ IU/mg in the presence of 50 pg/ml rRtIL-1α.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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