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产品描述:

产品名称:Recombinant Human G-CSF

氨基酸序列: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

参考文献: 1.Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.<br/>2.Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.<br/>3.Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.<br/>4.Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.<br/>5.Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.

Accession: <a href="http://www.uniprot.org/uniprot/P09919" target="_blank">P09919</a>

中文别名: G-CSF蛋白; CSF1蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/1440" target="_blank">1440</a>

种属: Human

来源: Escherichia coli.

纯度: > 98 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanG-CSF as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 10mM sodium acetate buffer, containing 5 % trehalose, pH 4.0.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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