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产品描述:

产品名称:Recombinant Human CYR61

氨基酸序列: TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD

参考文献: 1.Jay P, Berge-Lefranc JL, Marsollier C, et al. 1997. Oncogene. 14:1753-7.<br/>2.Lau LF. 2011. Cell Mol Life Sci. 68:3149-63.<br/>3.Jun JI, Lau LF. 2011. Nat Rev Drug Discov. 10:945-63.<br/>4.Holbourn KP, Acharya KR, Perbal B. 2008. Trends Biochem Sci. 33:461-73.

Accession: <a href="http://www.uniprot.org/uniprot/O00622" target="_blank">O00622</a>

中文别名: Cyr61蛋白; CCN1蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/3491" target="_blank">3491</a>

种属: Human

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanCYR61 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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