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产品描述:

产品名称:Recombinant Human TNF-α/TNFSF2, His

氨基酸序列: MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL

参考文献: 1.Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.<br/>2.Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.<br/>3.Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.<br/>4.Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.

Accession: <a href="http://www.uniprot.org/uniprot/P01375" target="_blank">P01375</a>

中文别名: TNF-α蛋白; TNFSF2蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/7124" target="_blank">7124</a>

种属: Human

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanTNF-α/TNFSF2, His as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg in the presence of actinomycin D.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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