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产品描述:

产品名称:Recombinant Rat IGF-1

氨基酸序列: GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA

参考文献: 1.Skottner A, Fryklund L, Hansson HA. 1986. Acta Paediatr Scand Suppl, 325: 107-11.<br/>2.Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.<br/>3.Palmade F, Sechoy-Chambon O, Coquelet C, et al. 1994. Curr Eye Res, 13: 531-7.<br/>4.Tennagels N, Hube-Magg C, Wirth A, et al. 1999. Biochem Biophys Res Commun, 260: 724-8.<br/>5.Laron Z. 2004. Novartis Found Symp, 262: 56-77; discussion -83, 265-8.<br/>6.Shiratsuchi I, Akagi Y, Kawahara A, et al. 2011. Anticancer Res, 31: 2541-5.

Accession: <a href="http://www.uniprot.org/uniprot/P08025" target="_blank">P08025</a>

中文别名: IGF-1蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/24482" target="_blank">24482</a>

种属: Rat

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rRtIGF-1 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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