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产品描述:

产品名称:Recombinant Rat IFN-γ

氨基酸序列: QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC

参考文献: 1.Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.<br/>2.Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.<br/>3.Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, <br/>4.Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.

Accession: <a href="http://www.uniprot.org/uniprot/P01581" target="_blank">P01581</a>

中文别名: IFN-γ蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/25712" target="_blank">25712</a>

种属: Rat

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 0.1 EU/μg of rRtIFN-γ as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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