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产品描述:

产品名称:Recombinant Hirudin

氨基酸序列: VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

参考文献: 1.Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.<br/>2.Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.<br/>3.Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.<br/>4.Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.<br/>5.Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.

Accession: <a href="http://www.uniprot.org/uniprot/P84590" target="_blank">P84590</a>

中文别名: Hirudin蛋白

种属: Invertebrate

来源: Pichia pastoris

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rHirudin as determined by LAL method.

活性: The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.

形态: Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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