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产品描述:

产品名称:Recombinant Rat IL-4

氨基酸序列: HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS

参考文献: 1.Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.<br/>2.Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.<br/>3.Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.<br/>4.Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.<br/>5.Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.<br/>

Accession: <a href="http://www.uniprot.org/uniprot/P20096" target="_blank">P20096</a>

中文别名: IL-4蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/287287" target="_blank">287287</a>

种属: Rat

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rRtIL-4 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20( C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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