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产品描述:

产品名称:Recombinant Rat GDNF

氨基酸序列: SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

参考文献: 1.Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2.<br/>2.Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80.<br/>3.Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7.<br/>4.Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.

Accession: <a href="http://www.uniprot.org/uniprot/Q07731" target="_blank">Q07731</a>

中文别名: GDNF蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/25453" target="_blank">25453</a>

种属: Rat

来源: Escherichia coli.

纯度: > 98 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 0.1 EU/μg of rRtGDNF as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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