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产品描述:

产品名称:Recombinant Human SHH

氨基酸序列: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

参考文献: 1.Feijoo CG, Onate MG, Milla LA, et al. 2011. Eur J Neurosci, 33: 589-98.<br/>2.Bear KA, Solomon BD, Roessler E, et al. 2012. Clin Dysmorphol, 21: 148-51.<br/>3.Mukhopadhyay A, Krishnaswami SR, Cowing-Zitron C, et al. 2012. Dev Biol, <br/>4.Zhang M, Wang H, Teng H, et al. 2010. Histochem Cell Biol, 134: 327-35.<br/>5.Bayly RD, Brown CY, Agarwala S. 2012. Dev Biol, 369: 32-42.

Accession: <a href="http://www.uniprot.org/uniprot/Q15465" target="_blank">Q15465</a>

中文别名: SHH蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/6469" target="_blank">6469</a>

种属: Human

来源: Escherichia coli.

纯度: > 98 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanSHH as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 × 10³ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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