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产品描述:

产品名称:Recombinant Mouse SHH

氨基酸序列: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

参考文献: 1.Currie PD, Ingham PW. 1996. Nature. 382:452-5.<br/>2.Herzog W, Zeng X, Lele Z, et al. 2003. Dev Biol. 254:36-49.<br/>3.Lewis KE, Eisen JS. 2001. Development. 128:3485-95.<br/>4.Scholpp S, Wolf O, Brand M, et al. 2006. Development. 133:855-64.<br/>5.Dassule HR, Lewis P, Bei M, et al. 2000. Development. 127:4775-85.<br/>6.Porter JA, Young KE, Beachy PA. 1996. Science. 274:255-9.

Accession: <a href="http://www.uniprot.org/uniprot/Q62226" target="_blank">Q62226</a>

中文别名: SHH蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/20423" target="_blank">20423</a>

种属: Murine

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of rMuSHH as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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