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产品描述:

产品名称:Recombinant Human SPARC, His

氨基酸序列: MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI

参考文献: 1.Sage H, Decker J, Funk S, et al. 1989. J Mol Cell Cardiol, 21 Suppl 1: 13-22.<br/>2.Kanauchi M, Nishioka M, Dohi K. 2000. Diabetologia, 43: 1076-7.<br/>3.Lau CP, Poon RT, Cheung ST, et al. 2006. J Pathol, 210: 459-68.<br/>4.Rodriguez-Jimenez FJ, Caldes T, Iniesta P, et al. 2007. Oncol Rep, 17: 1301-7.<br/>5.Wong SY, Crowley D, Bronson RT, et al. 2008. Clin Exp Metastasis, 25: 109-18

Accession: <a href="http://www.uniprot.org/uniprot/P09486" target="_blank">P09486</a>

中文别名: SPARC蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/6678" target="_blank">6678</a>

种属: Human

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanSPARC, His as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 µg/mL, corresponding to a specific activity of > 333 IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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