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产品描述:

产品名称:Recombinant Human IL-33

氨基酸序列: SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

参考文献: 1.Liew FY, Pitman NI, McInnes IB. 2010. Nat Rev Immunol, 10: 103-10.<br/>2.Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46.<br/>3.Bourgeois E, Van LP, Samson M, et al. 2009. Eur J Immunol, 39: 1046-55.<br/>4.Zhiguang X, Wei C, Steven R, et al. 2010. Immunol Lett, 131: 159-65.<br/>5.Turnquist HR, Zhao Z, Rosborough BR, et al. 2011. J Immunol, 187: 4598-610.

Accession: <a href="http://www.uniprot.org/uniprot/O95760" target="_blank">O95760</a>

中文别名: IL-33蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/90865" target="_blank">90865</a>

种属: Human

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanIL-33 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.

形态: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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