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产品描述:

产品名称:Recombinant Human FGF-4

氨基酸序列: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

参考文献: 1.Galland F, Stefanova M, Lafage M, et al. 1992. Cytogenet Cell Genet. 60:114-6.<br/>2.Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>3.Boulet AM, Capecchi MR. 2012. Dev Biol. 371:235-45.

Accession: <a href="http://www.uniprot.org/uniprot/P08620" target="_blank">P08620</a>

中文别名: FGF-4蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/2249" target="_blank">2249</a>

种属: Human

来源: Escherichia coli.

纯度: > 96 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanFGF-4 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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