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产品描述:

产品名称:Recombinant Human IL-7

氨基酸序列: DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

背景说明: Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection.

参考文献: 1.Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.<br/>2.Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.<br/>3.Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.<br/>4.Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.<br/>5.Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.<br/>6.Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.

英文别名: LP-1, pre-B cell factor

Accession: P13232

中文别名: IL-7蛋白

Gene IDs: 3574

来源: Escherichia coli.

分子量: Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

外观: Sterile Filtered White lyophilized (freeze-dried) powder.

内毒素水平: Less than 1 EU/μg of rHuIL-7 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) is 0.1-0.5 ng/mL. The specific activity of Recombinant Human IL-7 is approximately 2.0 × 108 units/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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