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产品描述:

产品名称:Recombinant Human aFGF

氨基酸序列: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

参考文献: 1.Dionne CA, Crumley G, Bellot F, et al. 1990. EMBO J. 9:2685-92.<br/>2.Jaye M, Howk R, Burgess W, et al. 1986. Science. 233:541-5.<br/>3.Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>4.Gospodarowicz D. 1974. Nature. 249:123-7.<br/>5.Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.<br/>6.Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>7.Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>8.Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>9.Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.

Accession: <a href="http://www.uniprot.org/uniprot/P05230" target="_blank">P05230</a>

中文别名: aFGF蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/2246" target="_blank">2246</a>

种属: Human

来源: Escherichia coli.

纯度: > 95 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanaFGF as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg in the presence of 10 μg/ml of heparin.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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