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产品描述:

产品名称:Recombinant Human NT-4

氨基酸序列: MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

参考文献: 1.Gao WQ, Zheng JL, Karihaloo M. 1995. J Neurosci, 15: 2656-67.<br/>2.Ogborn DIandGardiner PF. 2010. Muscle Nerve, 41: 385-91.<br/>3.Peinado-Ramon P, Salvador M, Villegas-Perez MP, et al. 1996. Invest Ophthalmol Vis Sci, 37: 489-500.<br/>4.Yuen ECandMobley WC. 1999. Exp Neurol, 159: 297-308.<br/>5.Sakuma K, Watanabe K, Sano M, et al. 2001. Brain Res, 907: 1-19.

Accession: <a href="http://www.uniprot.org/uniprot/P34130" target="_blank">P34130</a>

中文别名: NT-4蛋白

Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/4909" target="_blank">4909</a>

种属: Human

来源: Escherichia coli.

纯度: > 97 % by SDS-PAGE and HPLC analyses.

内毒素水平: Less than 1 EU/μg of Recombinant HumanNT-4 as determined by LAL method.

活性: Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg.

形态: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5.

溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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