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抗体描述:

产品名称:Rabbit anti-Histone H3K27me3 Polyclonal Antibody

产品别名:Histone H3K27me3抗体  

宿主:Rabbit

反应种属:Human;Mouse;Rat

克隆性:Polyclonal Antibody

保存方法:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

产品描述:Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

用途范围: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

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