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- 品牌：爱必信(absin)
- 产地：上海
- 型号：50ul
- 货号：abs110296
- 价格： ￥1500/支
- 发布日期： 2021-12-30
- 更新日期： 2024-04-26
| 产地 | 上海 |
| 品牌 | 爱必信(absin) |
| 保存条件 | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 货号 | abs110296 |
| 应用范围 | WB~~1:1000<br />IHC-P~~1:10~50<br />FC~~1:10~50 |
| 抗原来源 | 见爱必信官网 |
| 保质期 | 见爱必信官网 |
| 规格 | 50ul |
| 产品用途 | 见爱必信官网 |
| 抗体名 | DLAT抗体 |
| 靶点 | DLAT |
| 适应物种 | 见爱必信官网 |
| 用途范围 | WB~~1:1000<br />IHC-P~~1:10~50<br />FC~~1:10~50 |
| 形态 | 见爱必信官网 |
| 宿主 | Rabbit |
| 标记物 | 见爱必信官网 |
| 亚型 | 见爱必信官网 |
| 标识物 | 见爱必信官网 |
| 浓度 | -% |
| 免疫原 | 见爱必信官网 |
| 是否进口 | 否 |
| 概述 | |
| 描述 |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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| 别名 |
DLAT抗体;Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, DLAT, DLTA,DLAT,DLTA
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| 宿主 |
Rabbit
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| 特异性 |
This DLAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 579-607 amino acids from the C-terminal region of human DLAT.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:10~50 FC~~1:10~50 |
| 分子量 |
Predicted molecular weight: 69kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
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| 同种型 |
Rabbit Ig
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| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Cancer;Metabolism;Signal Transduction
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| 靶标 | |
| 背景说明 |
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
|
| 细胞定位 |
Mitochondrion matrix.
|
| UniProt |
P10515
|
| 参考文献 | |
| 参考文献 |
Trynka, G., et al. Gut 58(8):1078-1083(2009)
Lleo, A., et al. Hepatology 49(3):871-879(2009) Korotchkina, L.G., et al. FEBS Lett. 582(3):468-472(2008) |
实验结果图
All lanes : Anti-DLAT Antibody (C-term) at 1:1000 dilution Lane 1: LNCaP whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
DLAT Antibody (C-term) (Cat. #abs110296) western blot analysis in K562(lane 1),HepG2(lane 2),Jurkat(lane 3) cell line lysates (35ug/lane).This demonstrates the DLAT antibody detected the DLAT protein (arrow).
DLAT Antibody (C-term) (Cat. #abs110296) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DLAT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
DLAT Antibody (C-term) (Cat. #abs110296) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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