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产品描述:

产品名称:细胞增殖检测试剂盒(MTS)

中文别名: Cell Proliferation Assay Kit(MTS)

描述:

Cell Proliferation Assay (MTS)以比色法检测细胞增殖、细胞毒性或化学敏感性分析中的活细胞数量。本试剂包含一种四唑化合物[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)- 2H-tetrazolium, 内盐;MTS] 和一个电子耦合试剂( 乙硫吩嗪,PES)。与PMS 相比,PES 的化学稳定性更强,能与MTS 混合形成单一稳定的溶液。进行检测时,只需将少量的本试剂直接加入到培养孔中,孵育1-4 小时,然后在96 孔板读板仪上记录490nm 的吸光度。在490nm 测量的甲产物的吸光度值与培养物中活细胞的数量直接成正比。

使用方法: 1. Thaw the Reagent. It should take approximately 90 minutes at room temperature, or 10 minutes in a water bath at 37°C, to completely thaw the 20ml size. 2. Pipet 20μl of Reagent into each well of the 96-well assay plate containing the samples in 100μl of culture medium. Note: We recommend repeating pipettes, digital pipettes or multichannel pipettes for convenient delivery of uniform volumes of Reagent to the 96-well plate. 3. Incubate the plate at 37°C for 1–4 hours in a humidifi ed, 5% CO2 atmosphere. Note: To measure the amount of soluble formazan produced by cellular reduction of MTS, proceed immediately to Step 4. Alternatively, to measure the absorbance later, add 25μl of 10% SDS to each well to stop the reaction. Store SDS-treated plates protected from light in a humidifi ed chamber at room temperature for up to 18 hours. Proceed to Step 4. 4. Record the absorbance at 490nm using a 96-well plate reader.

储存/保存方法: 2-8℃避光保存。

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