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试剂描述:

产品名称:考马斯亮蓝G250

产品别名:酸性蓝90;考马斯亮蓝G

CAS号:6104-58-1

英文别名:考马斯亮蓝G250

分子量:854.02

分子式:C₄₇H₄₈N₃O₇S₂•Na

纯度:

外观:见爱必信官网

保存方法:常温,避光防潮密闭干燥,有效期3年。

描述:

Brilliant Blue G (CBBG) is a member of the Coomassie dye family and is commonly used in analytical biochemistry to stain proteins in SDS-PAGE and BN-PAGE. CBBG is a useful component in the Bradford Method, a dye-binding protein assay employed to determine protein concentration in a given solution. CBBG can exist in three forms: a cation (red form with maximum absorbance at 470 nm), a neutral form (green with maximum at 650 nm), and an anionic form (blue with maximum at 595 nm). The anionic form initiates a stable complex with basic amino acid residues, mainly arginine and aromatic amino acids. Although the anionic form exists in negligible concentrations at the assay reaction mixture's pH, the protein-binding shifts the equilibrium toward its formation. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.

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