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试剂描述:

产品名称:咪唑

产品别名:1,3-二氮唑; 间二氮茂; 甘恶啉; IMZ; 1,3-二氨杂环戊二烯; 1,3-二氮杂环戊二烯; 1,3-二氮杂茂; 1,3-二氮杂-2,4-环戊二烯

CAS号:288-32-4

英文别名:咪唑

分子量:68.08

分子式:C₃H₄N₂

纯度:99%

外观:见爱必信官网

保存方法:室温,有效期3年。

描述:

Imidazole is useful as a buffer in the pH range of 6.2-7.8 One of the applications of imidazole is in the purification of His-tagged proteins in immobilised metal affinity chromatography(IMAC). Imidazole is used to elute tagged proteins bound to Ni ions attached to the surface of beads in the chromatography column. An excess of imidazole is passed through the column, which displaces the His-tag from nickel co-ordination, freeing the His-tagged proteins. Imidazole has become an important part of many pharmaceuticals. Synthetic imidazoles are present in many fungicides and antifungal, antiprotozoal, and antihypertensive medications. Imidazole is part of the theophylline molecule, found in tea leaves and coffee beans, which stimulates the central nervous system. It is present in the anticancer medication mercaptopurine, which combats leukemia by interfering with DNA activities.

应用:A buffer useful in the pH range of 6.2-7.8

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