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抑制剂描述:

产品名称:Rolipram

产品别名:见爱必信官网

英文别名:Rolipram

靶点:PDE

CAS:61413-54-5

纯度:>98%

外观:见爱必信官网

保存方法:Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.

描述:

Rolipram(SB95952; ZK62711)是磷酸二酯酶PDE4选择性抑制剂,对PDE4B和PDE4D的IC₅₀分别为130nM和240 nM。

溶解性:DMSO : 20.7 mg/mL (75 mM)
Ethanol : 20.7 mg/mL (75 mM)

体外研究:

The PDE4 selective inhibitor, Rolipram, inhibits immunopurified PDE4B and PDE4D activities similarly, with IC₅₀s of approx. 130 nM and 240 nM respectively. In contrast, Rolipram inhibits immunopurified PDE4A activity with a dramatically lower IC₅₀ of around 3 nM. Rolipram increases phosphorylation of cAMP-response-element-binding protein (CREB) in U937 cells in a dose-dependent fashion, which implies the presence of both high affinity (IC₅₀ approx. 1 nM) and low affinity (IC₅₀ approx. 120 nM) components. Rolipram dose-dependently inhibits the IFN-gamma-stimulated phosphorylation of p38 MAPK in a simple monotonic fashion with an IC₅₀ of approx. 290 nM. Rolipram is a selective PDE4 inhibitor that inhibits all PDE4 isoforms A, B, C and D. Rolipram inhibits LPS-induced TNF production in a dose-dependent manner (IC₅₀ 25.9 nM), and maximal/submaximal inhibition is observed with 2 μM drug concentration in J774 cells.

体内研究:TNF mRNA and protein expression is induced by LPS in peritoneal macrophages (PM) from WT mice, and that is clearly (by 74 and 63% for TNF mRNA and TNF protein, respectively) inhibited by Rolipram. LPS-induced TNF production is enhanced in PM from MKP-1(-/-) mice as compared to that in PM from WT mice, which is in line with the published results. Interestingly, the inhibition of TNF mRNA and protein expression by Rolipram is markedly attenuated in PM from MKP-1(-/-) mice and does not reach statistical significance. Repeated administration of Rolipram (1.25 mg/kg, i.p.) reduces the number of escape failures in learned helplessness rats.

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